CD146 and its Soluble Form Regulate Monocyte Transendothelial Migration

CD146 and its soluble form regulate monocyte transendothelial migration.

OBJECTIVES During inflammation, cell adhesion molecules are modulated or redistributed for leukocyte transmigration. Among molecules at the interendothelial junction, CD146 is involved in cell-cell cohesion and permeability, but its role in monocyte transmigration is unknown. METHODS AND RESULTS TNF enhanced CD146 expression at the junction and apical membrane of human umbilical veins endothe...

Monocyte Transendothelial Migration: Lipophosphoglycan Reduces Leishmania

Endothelial CD99 signals through soluble adenylyl cyclase and PKA to regulate leukocyte transendothelial migration

CD99 is a critical regulator of leukocyte transendothelial migration (TEM). How CD99 signals during this process remains unknown. We show that during TEM, endothelial cell (EC) CD99 activates protein kinase A (PKA) via a signaling complex formed with the lysine-rich juxtamembrane cytoplasmic tail of CD99, the A-kinase anchoring protein ezrin, and soluble adenylyl cyclase (sAC). PKA then stimula...

The Soluble and Particulate Form of Alginates Positively Regulate Immune Response

Background: Alginate materials have been widely employed for biomedical applications ranging from wound healing to cancer treatment. However, how alginate materials affect the immune system is largely unknown. Objective: To explore the impact of alginate materials on immune system. Methods: The effect of three types of alginate materials, low viscosity, high viscosity and particulate alginate, ...

RhoA is required for monocyte tail retraction during transendothelial migration

Transendothelial migration of monocytes is the process by which monocytes leave the circulatory system and extravasate through the endothelial lining of the blood vessel wall and enter the underlying tissue. Transmigration requires coordination of alterations in cell shape and adhesive properties that are mediated by cytoskeletal dynamics. We have analyzed the function of RhoA in the cytoskelet...